| 摘要 |
<p>PURPOSE:To provide a process for culturing human hair cell having proliferation and differentiation capability and useful for the development of hair-growing and hair-tonic agent and the investigation of hair, etc., especially a process suitable for the separation and culture of scalp hair tissue and to provide a culture medium suitable for the above process. CONSTITUTION:Hair tissue is separated in a damage-free state from the epidermic tissue and/or the dermic tissue of a collected human skin piece by an enzymatic treatment including dispase treatment, collagenase treatment and trypsin treatment. The hair tissue is cultured in a medium for hair cell containing amino acids (L-histidine, L-leucine, L-methionine, L-phenylalanine, tryptophan and L-tyrosine) in an amount corresponding to 1.3-4 times the amount in conventional medium. The pure culture of hair cell including the inner epidermal sheath cell can be carried out by this process.</p> |
