发明名称 |
Production of recombinant proteins using herpes virus promoters and VP16 transactivators. |
摘要 |
<p>Stable cell lines are produced to express high levels of a gene product of interest using VP16, a herpes simplex virus transactivator, and a promoter from herpes simplex virus which is a target for VP16. The transactivator and promoter are introduced to a cell line separately using antibiotic resistance genes as selectable markers on separate vectors.</p> |
申请公布号 |
EP0566554(A2) |
申请公布日期 |
1993.10.20 |
申请号 |
EP19930870043 |
申请日期 |
1993.03.12 |
申请人 |
MONSANTO COMPANY |
发明人 |
HIGHKIN, MAUREEN KATHERINE;HIPPENMEYER, PAUL JEROME |
分类号 |
C12N5/10;C07K14/52;C07K14/61;C07K14/705;C12N9/10;C12N9/72;C12N15/09;C12N15/869;C12P21/02;C12R1/91;G01N33/50;(IPC1-7):C12N15/85;C12N7/02;C12Q1/18 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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