SERUM FREE TISSUE CULTURE MEDIUM CONTAINING POLYMERIC CELL-PROTECTIVE AGENT

摘要

An essentially serum-free tissue culture medium characterised in that it includes in solution a polymer which acts as cell protective agent by reducing film drainage around cells. Preferred polymers are polyethylene glycol, polyvinyl pyrollidone and polymers containing one or more alkylene oxide. This tissue culture medium is of particular use in the cultivation of cells requiring attachment under aeration. Methods of cultivation of such cells are also claimed.