| 摘要 |
<p>PURPOSE:To readily peel and recover spheroidal hepatic cells from a cell culture support in a high yield without damaging the cells and without being mixed or contaminated with the third component such as a chemical by culturing the hepatic cells in the spheroidal state and subsequently peeling and recovering the hepatic cells cultured in the spheroid state only by changing the circumferential temperature. CONSTITUTION:Hepatic cells are cultured at <= an upper limit critical dissolution temperature or at >= a lower limit critical dissolution temperature on a cell culture support produced by coating a polymer having the critical dissolution temperature of 0-80 deg.C in water on the surface of a substrate, and subsequently subjected to >= the upper limit critical dissolution temperature or <= the lower limit critical dissolution temperature.</p> |
