摘要 |
PURPOSE:To obtain the subject compound useful as a production raw material for poly-3-hydroxybutyrate, etc., useful as a biodegradable polymer on an industrial scale at a low cost by using a cultured product of a microbial strain belonging to the genus Bacillus or treated cell of the microbial strain. CONSTITUTION:3-Hydroxybutyric acid and/or its trimer useful as a production raw material for biodegradable polymer, etc., are produced by the following microbial reaction and/or enzymatic reaction. A microbial strain belonging to the genus Bacillus and capable of producing 3-hydroxybutyric acid and/or its trimer [e.g. Bacillus megatherium B-124 (FERM P-12803)] or its treated cell is added to a liquid medium consisting of sucrose, NH4Cl, NaCl, K2HPO4, KH2PO4, MgSO4.7H2O, corn steep liquor and distilled water and cultured at 30 deg.C under shaking, the cultured product is extracted with ethyl acetate and the solvent is distilled off from the extracted liquid. |