| 摘要 |
PURPOSE:To remarkably stably produce a large quantity of D-ribose by modifying a D-ribose-producing bacterium so as to enable high-manifestation of the DNA sequence contributing to manifestation of gluconic acid operon in a Bacillus bacterium, culturing the modified bacterium in a culture medium and collecting the product. CONSTITUTION:In modifying a part or all of the DNA sequence contributing to manifestation of gluconic acid operon so as to enable a high-manifestation of the gluconic acid operon in a Bacillus bacterium, the modification is carried out by deactivation, e.g. due to missing of gntR part as the manifestation control region existing on the upper stream side of an open reading frame coding gluconokinase of gluconic acid operon or due to insertion of another DNA into his manifestation control region and/or by substituting the promoter part with a Bacillus bacterium chromosomal DNA-derived promoter or a Bacillus bacterium phage-derived promoter, so as to obtain a D-ribose-producing Bacillus bacterium. The resultant D-ribose-producing Bacillus bacterium is cultured in a culture medium and its product is accumulated. The accumulated product is collected, thus remarkably stably producing a large quantity of D-ribose. |
