Method for kit for estimating the amount of methyl anthranilate

摘要

A method and kit for estimating the amount of methyl anthranilate includes obtaining a sample suspected of having methyl anthranilate, acidifying the sample with a strong acid, adding sodium nitrite to the acidified sample producing nitrous acid which in the presence of methyl anthranilate produces a diazonium ion. The method further comprises a step of adding sulfamic acid which removes excess nitrous acid from the sample, followed by the addition N-(1-naphthyl) ethylenediamine which reacts with the diazonium ion to form a colored complex which is color responsive which is then compared to standards prepared using known amounts of methyl anthranilate. The kit provides the a sample holder and vials of ingredients and standards to practice the method.