Specifically beta - beta cross-linked hemoglobins and method of preparation

摘要

A modified hemoglobin comprising hemoglobin which is cross-linked with a cross-linking reagent. The cross-linking reagent is selected such that the beta -chains are cross-linked within the 2,3-diphosphoglycerate binding site and the linkage distance between the beta -chains is between about 5 to 9 angstroms. A method of preparing the modified hemoglobin and its use as a blood substitute or a plasma expander are also described.