| 摘要 |
PCT No. PCT/DK92/00105 Sec. 371 Date Sep. 23, 1993 Sec. 102(e) Date Sep. 23, 1993 PCT Filed Apr. 3, 1992 PCT Pub. No. WO92/17578 PCT Pub. Date Oct. 15, 1992.An alkaline protease from a strain of Bacillus sp. JP 395 has been isolated. The protease has a mass of about 30 kDa as determined by SDS-PAGE, a pH optimum of about 9-11, a temperature optimum of about 50 DEG -65 DEG C., and an isoelectric point above 9.5. The protease is inhibited by PMSF (phenylmethylsulfonylfluoride), but not by EDTA or soybean protein inhibitor. The protease is prepared by growing this strain of bacillus in a suitable media and recovering the protease. The protease has been formulated in detergents and is stable at 40 DEG C. for 60 minutes in these detergents under wash conditions in the presence or absence of bleaches such as perborate.
|
