| 摘要 |
A method of cloning foreign DNA into a DNA vector comprising ligating: (1) a DNA vector having a single stranded DNA overhang at each end, said overhangs being mutually incompatible so as to prevent self-religation, with (2) a linear piece of foreign DNA having a single stranded DNA overhang at each end, each foreign DNA overhang being complementary to but at least one base shorter than each of the vector overhangs and being capable of base pairing along the entire length of the overhang with one of the vector overhangs, and sealing the gap by either transforming the double stranded DNA having a gap therein into a suitable bacterium or transfecting it into a suitable bacterium after packaging it into a suitable bacteriophage. |
