发明名称 |
A METHOD FOR THE SIMULTANEOUS DETERMINATION OF DNA SEQUENCE VARIATIONS AT A LARGE NUMBER OF SITES, AND A KIT THEREFOR |
摘要 |
This invention relates to a method of simultaneously determining DNA sequence variations at a large number of sites, and a kit suitable therefor. The method comprises an electrophoretic separation of restriction fragments in two dimensions on the basis of two independent criterions, namely, length and base pair sequence, and a detection of separated fragments by means of a labelled probe comprising one or more cores of repeat sequences. Preferred probes are GC-rich minisatellite or simple sequence core sequences. |
申请公布号 |
EP0349024(B1) |
申请公布日期 |
1993.09.29 |
申请号 |
EP19890201095 |
申请日期 |
1989.04.28 |
申请人 |
NEDERLANDSE ORGANISATIE VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK TNO |
发明人 |
VIJG, JAN;UITTERLINDEN, ANDREAS GERARDUS |
分类号 |
G01N27/447;C12Q1/68 |
主分类号 |
G01N27/447 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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