发明名称 |
METHODS FOR GENERATING LIGHT WITH CHEMILUMINESCENT DIOXETANES ACTIVATED BY ANCHIMERIC ASSISTED CLEAVAGE |
摘要 |
Novel assay methods employing compounds which are chemically or enzymatically cleavable and which give rise to an intermediate which further decomposes by an intramolecular anchimeric displacement reaction which releases a signal producing species are disclosed. Also disclosed are probe hybridization assays employing the compounds of the invention employing thermostable enzymes which are not denatured by the hybridization conditions. Such signal producing species may include chemiluminescent dioxetanes and other colored products.
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申请公布号 |
US5248618(A) |
申请公布日期 |
1993.09.28 |
申请号 |
US19910710332 |
申请日期 |
1991.06.05 |
申请人 |
LIFE TECHNOLOGIES, INC. |
发明人 |
HACES, ALBERTO |
分类号 |
C07F9/10;C07F9/655;G01N33/535 |
主分类号 |
C07F9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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