| 发明名称 |
LABELING BY SIMULTANEOUS LIGATION AND RESTRICTION |
| 摘要 |
<p>Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of the restriction enzyme recognition sequence.</p> |
| 申请公布号 |
EP0327429(B1) |
申请公布日期 |
1993.09.22 |
| 申请号 |
EP19890400220 |
申请日期 |
1989.01.26 |
| 申请人 |
APPLIED BIOSYSTEMS, INC. |
发明人 |
KEITH, DOUGLAS H.;KRONICK, MEL N.;MCBRIDE, LINCOLN J.;WHITELEY, NORMAN M. |
| 分类号 |
C07H21/04;C12N15/09;C12Q1/68;C12Q1/70;G01N33/50 |
主分类号 |
C07H21/04 |
| 代理机构 |
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代理人 |
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| 主权项 |
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| 地址 |
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