发明名称 |
Thiobenzoylation method of peptide sequencing with gas chromatography and mass spectrometric detection |
摘要 |
A protein microsequencing method for use in conjunction with the thiobenzoylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thiobenzoylating reagent. The derivatized polypeptide is subjected to cleavage by acid which forms a 4-substituted 2-phenyl-5(4H) thiazolone. The thiazolone is acylated to form a 5-acyloxy-2-phenylthiazole and subjected to detection by both gas chromatography and chemical ionization mass spectroscopy.
|
申请公布号 |
US5246865(A) |
申请公布日期 |
1993.09.21 |
申请号 |
US19920919524 |
申请日期 |
1992.07.24 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
STOLOWITZ, MARK L. |
分类号 |
C07K1/12;G01N30/02;G01N33/68 |
主分类号 |
C07K1/12 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|