摘要 |
The present invention concerns the production of useful proteins to the growth medium of yeast by using the hsp150 protein or fragments or derivates thereof as a carrier. The authentic hsp150 protein, encoded by the H &cir& _S &cir& _P &cir& _150 gene of Saccharomyces cerevisiae, is secreted efficiently to the growth medium. In the new recombinant DNA vectors the gene encoding the desired protein is joined to the H &cir& _S &cir& _P &cir& _150 nucleotide sequence. The fusion gene is transformed to a yeast host to express the respective fusion protein. The signal sequence of the hsp150 protein, or another signal sequence, guides the fusion protein to the secretory pathway, and the hsp150-carrier takes it to the medium, from where it is harvested. During secretion, the protein product may or may not be detached from the hsp150 carrier by proteolytic enzymes, according to the occurrence of natural or created cleavage sites at the carrier-product junction. |