发明名称 |
Cloned genes encoding reverse transcriptase lacking RNase H activity |
摘要 |
The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.
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申请公布号 |
US5244797(A) |
申请公布日期 |
1993.09.14 |
申请号 |
US19910671156 |
申请日期 |
1991.03.18 |
申请人 |
LIFE TECHNOLOGIES, INC. |
发明人 |
KOTEWICZ, MICHAEL L.;GERARD, GARY F. |
分类号 |
C07H21/04;C07K14/16;C12N7/00;C12N7/01;C12N9/12;C12N9/22;C12N15/54;C12P19/34;C12Q1/68;C12Q1/70 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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