| 摘要 |
PURPOSE:To produce the subject enzyme improved in a relative productivity of gamma-CD to beta-CD in comparison with wild type CGTase by substituting the 188th tyrosine of the sequence No.1 amino acid sequence derived from a specified microorganism with alanine, etc. CONSTITUTION:The 1756th to 1758th TAT in the sequence No.1 base sequence of a DNA of Bacillus ohbensis sp. nov. C-1400 are substituted with GCT, GCC, GCA, GCG, TCT, etc., and the resultant modified cyclodextrin glucanotransferase (CGTase) gene is subsequently introduced into a microorganism. The obtained transformat microorganism is then cultured and the objective CGTase in which the 188th tyrosine of the sequence No.1 amino acid sequence is substituted with alanine, serine or tryptophan can be obtained from the resultant culture solution. |
