| 摘要 |
A novel omega -carboxyalcohol oxidase catalyzes at least one of the following reactions: R-CH2OH+O2->R-CHO+H2O2 a) R-CHO+O2+H2O->R-COOH+H2O2 b) wherein R is alkyl, alkenyl, omega -carboxyalkyl or omega -carboxyalkenyl. The enzyme has substrate specificity on at least 12-hydroxydodecanoic acid, 1-dodecanol, 1-decanol, 1-octanol and 1-hexanol, and has no substrate specificity on methanol, ethanol or glycerol. The enzyme does not require the presence of NAD or NADP for its use. Also disclosed is a process for producing the enzyme, an assay method for substrates of the enzyme, and a process for producing carboxylic acid employing the enzyme.
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