发明名称 |
HOMOGENOTIZATION OF GENE-TARGETING EVENTS |
摘要 |
Homozygotic cells are obtained by employing homologous recombination with a construct comprising a marker gene. The marker gene allows for selection without amplification and by employing elevated levels of the antibiotic to which the marker gene imparts resistance, gene conversion can occur, where in a diploid host, both copies of the target locus will be the same. In this manner, knock-outs of genes can be readily achieved without requiring two steps of homologous recombination. |
申请公布号 |
WO9316177(A1) |
申请公布日期 |
1993.08.19 |
申请号 |
WO1993US00926 |
申请日期 |
1993.02.02 |
申请人 |
CELL GENESYS, INC.;THE PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
SEIDMAN, JONATHAN, G.;JAKOBOVITS, AYA |
分类号 |
A01K67/027;A61K48/00;C12N5/10;C12N15/09;C12N15/65;C12N15/85;C12N15/90 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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