发明名称 METHOD AND MATERIALS FOR DETERMINING PARTICLE COUNT IN A FLOW CYTOMETER
摘要 <p>A method is provided to ascertain the total number of cells per unit volume of a cell specimen in an apparatus which does not measure the volume of a suspension containing the specimen as it is being analyzed. The method comprising mixing a suspension of a known quantity of particles having a first light scatter signal with a known volume of a cell specimen having a second light scatter signal different from the first signal to obtain a suspension having a concentration of particles per specimen volume, passing each of said particles and each cell in said cell specimen, in turn, through a light beam, each of said particles and cells producing at least one forward light scattering pattern, counting the number of cells and the number of particles in a portion of said suspension, and determining the total number of cells per unit volume of specimen.</p>
申请公布号 WO1993016385(A1) 申请公布日期 1993.08.19
申请号 US1993001232 申请日期 1993.02.11
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