发明名称 |
SHEEP LFA-3 AND TM REGION-DELETED LFA-3 PROTEIN |
摘要 |
PURPOSE:To provide sheep LFA-3 protein which has high affinity to human T-cells, thus being useful as a diagnostic reagent for detecting T-cells, by culturing the cells transformed with a vector including the DNA sequence coding the sheep LFA-3 and collecting the product after cultivation. CONSTITUTION:RNA is extracted from sheep leukocytes with guanidine thiocyanate and the extract is treated with oligo (dT) cellulose column to isolate mRNA and cDNA is synthesized using it as a template and further prepare cDNA library. Then cloning is effected using it as a probe to collect the gene encoding sheep LFA-3 protein. The gene is multiplied by the PCR(polymerase- chain reaction) process and treated with a restriction enzyme to link to a suitable vector and introduced into host cells such as Escherichia coli and transformed. The transformed cells are cultured in a medium to enable high- efficient production of the objective sheep LFA-3 protein in high efficiency. |
申请公布号 |
JPH05199893(A) |
申请公布日期 |
1993.08.10 |
申请号 |
JP19920142795 |
申请日期 |
1992.06.03 |
申请人 |
KANEGAFUCHI CHEM IND CO LTD |
发明人 |
YAMASHITA KENJI;OOHARA TAKAAKI;NIWA HIDEO;KOSAKATA FUMIO;FUKUCHI TAKESHI;EDAMURA TADAHIRO;SUMIYA TORU |
分类号 |
C07K14/705;C07K14/00;C12N15/09;C12N15/12;C12P21/02;C12Q1/68;C12R1/19 |
主分类号 |
C07K14/705 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|