DYE-PROVIDING COMPOSITION, DIAGNOSTIC TEST KIT AND THEIR USE IN METHOD FOR LIGAND DETERMINATION USING PEROXIDASE LABELED-RECEPTOR

摘要

DYE-PROVIDING COMPOSITION, DIAGNOSTIC TEST KIT AND THEIR USE IN METHOD FOR LIGAND DETERMINATION USING PEROXIDASE LABELED-RECEPTOR A dye-providing composition comprises a water-soluble or -dispersible polymer, such as a vinylpyrrolidone polymer, and an imidazole leuco dye capable of providing a dye in the presence of hydrogen peroxide and a peroxidative substance. The weight ratio of polymer to leuco dye is from about 10,000:1 to about 100:1. The dye-providing composition can be included with a peroxidase substrate in a diagnostic test kit. A method for the determination of a ligand can be carried out using a peroxidase labeled-receptor for the ligand and the dye-providing composition described above. The method is particularly useful for the determination of human chorionic gonadotropin (hCG).