EXPRESSION OF OSTEOGENIC FACTOR OP-1 IN CELLS OF SPODOPTERA FRUGIPERDA INFECTED WITH RECOMBINANT BACULOVIRUS

摘要

Provided is a method for producing a DNA sequence encoding human osteogenic protein, OP-1, by recombinant DNA techniques. This sequence is employed in the preparation of recombinant baculovirus, which can be used to infect cells of Spodoptera frugiperda. Culturing such infected cells results in the production of physiologically active OP-1 protein, which can be employed to produce pharmaceutical compositions useful in the treatment of bone diseases, orthopedic diseases, bone defects, and trauma. Also provided are a recombinant baculovirus containing DNA encoding human OP-1, and insect cells infected with this recombinant baculovirus.