Fluorogenic and chromogenic betalactamase substrates.

摘要

Chromogenic and fluorogenic substrates for beta -lactamase, methods for synthesis thereof and methods for detecting beta -lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent beta -lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by beta -lactamase, the leaving group is liberated and fluorescence or a colored product is produced.