A high speed fluorescent emission imaging digital microscope has an Argon-Ion UV laser illumination source and a high speed optical filtering wheel in the illumination path to alternately select between two UV illumination wavelengths to produce fluorescent emission images at each UV wavelength. A dichroic reflector directs emission images along an image path and an galvanometer rotatable mirror exposes each image on a predetermined area of a CCD. A computer controls the rotatable mirror in response to exposure timing information from the filtering wheel. An objective lens is focused on the specimen for producing an image from a particular focal plane of the specimen. The objective lens is mounted on a counterweighted piezoelectric objective translator for changing the focal plane of the specimen image in response to control signals from the electronic processor.
申请公布号
US5233197(A)
申请公布日期
1993.08.03
申请号
US19910729978
申请日期
1991.07.15
申请人
UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER
发明人
BOWMAN, DOUGLAS;FAY, FREDRIC;RODGERS, CYRIL;TUFT, RICHARD