| 摘要 |
<p>PURPOSE:To suppress the ability to produce hydrogen sulfide and enable obtaining of beer in a short time by transducing a gene (NHS5) capable of exhibiting a prescribed base sequence suppressing the production of the hydrogen sulfide with a yeast. CONSTITUTION:A plasmid (pHS5), capable of suppressing H2S and having a well-known gene capable of suppressing H2S is subcloned to prepare a gene (NHS5) that is a protein having an amino acid sequence composed of a sequence of bases, etc., expressed by the formula in which the interval between prescribed positions is coded in the base sequence. The resultant NHS5 is then treated with a restriction enzyme to afford a plasmid (e.g. YCpAG-5SS), which is subsequently transduced into a yeast of the genus Saccharomyces for brewing to provide a transformant. The obtained transformant is then inoculated into a wort containing a hop added thereto and fermented to reduce the amount of the produced hydrogen sulfide from the parent strain by, 20-40%.</p> |
