Listeria monocytogenes detection by enzymatic nucleic acid amplification - using oligo-nucleotide(s) derived from alpha-haemolysin and/or beta-haemo-lysin virulence factors in polymerase chain reactions

摘要

Detection method for Listeria (L.) monocytogenes (mono.) comprises using the enzymatic nucleic acid amplification of hly (alpha-haemolysin) and/or iap (beta-haemolysin) virulence factors. Pref. oligonucleotides (A) L01=5'-CGGAGGTTCCGCAAAAGATG-3'; L02=5'-CATCGACGGCAACCTCGGA-3'; L03=5'-CCATCTGTATAAGCTTTTGAAG-3'; and L04=5'-CCTCCAGAGTGATCGATGTT-3' are used for hly and oligonucleotides (B) AD03=5'-ACAAGCTGCACCTGTTGCAG-3'; AD07=5'-TGACAGCGTGTGTAGTAGTCA-3'; AD08=5'-GGCGCAGGTGTAGTTGCTTG-3' and AD09=5'-CTACACAAGCAACTACACCT-3' are used for iap. USE/ADVANTAGE - L. mono can be detected in food samples by the oligonucleotides A and B. C.f. the classic bacteriological methods, the method is very fast (1-2 days) and sensitive thereby reducing any risk during the handling of L. mono. and avoiding possible genetic changes during the cultivation, e.g. the loss of virulence features.