SIZE-CALIBRATION DNA FRAGMENT MIXTURE AND METHOD

摘要

A size-calibration mixture for use in achieving single basepair resolution of polynucleotide fragments fractionated by high-resolution electrophoresis. The mixture includes two or more polynucleotide strand pairs, each pair having one strand which has a selected number of polynucleotide bases and a detectable reporter in its 5'-end region and a complementary strand which lacks the reporter. Also disclosed are methods of producing and using the mixture.