CHIMAERE ENZYME.

摘要

Chimeric alpha-amylases are disclosed of the formula Q-R-L in which Q is a N-terminal polypeptide residue of from 55 to 60 amino acid residues which is at least 75 percent homologous to the 55 N-terminal amino acid residues in the Bacillus amyloliquefaciens alpha-amylase as described in Takkinen et al., J. Biol. Chem. 258 (1983) 1007-1013, R is a polypeptide residue of the general formula in which X8 comprises His or Gln, X9 comprises Gly or Ser, and X10 comprises Ser or Asp; and L comprises a C-terminal polypeptide of from 390 to 400 amino acid residues which is at least 75 percent homologous to the 395 C-terminal amino acid residues in the Bacillus licheniformis 584 (ATCC 27811) alpha-amylase.