ENZYMATIC SYNTHESIS OF ISOTOPICALLY LABELED CARBOHYDRATES, NUCLEOTIDES AND CITRIC ACID INTERMEDIATES

摘要

The present invention relates to a general method of enzymatic synthesis of isotopically labeled carbohydrates, sugars and nucleosides. Labeled citric acid cycle intermediates, amino acids and ribose mononucleotides may be rapidly and conveniently synthesized from labeled pyruvate, lactate or L-alanine. The method employs a novel nicotinamide dinucleotide regeneration system which permits use of low NADH levels. The method may be manipulated to allow labeling at a variety of carbon/hydrogen sites.