| 摘要 |
2126438 9313216 PCTABS00024 The invention pertains to a method of site-specific mutagenesis of DNA. In this method, a parental DNA containing a non-essential, unique restriction site is used to generate progeny DNA containing the desired mutation but lacking the restriction site. The non-mutant parental DNA and the mutant progeny DNA are treated with any enzyme that cleaves at the restriction site to cleave the parental DNA, leaving the mutant DNA uncleaved. Mutant progeny DNA is selected by transforming cells with the enzyme-treated DNA under conditions in which the cells are transformed with uncleaved DNA at a higher efficiency than with cleaved DNA so that the majority of transformants carry the mutant DNA.
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