| 摘要 |
A hematological specimen for classifying and counting leukocytes with a flow cytometer is prepared. A sample to be assayed is prepared by eliminating influences of erythrocytes from a hematological sample without changing leukocytes morphologically by adding a first aqueous solution of a low osmotic pressure comprising a buffer for adjusting the pH value within an acidic region and a second aqueous solution comprising an osmolarity compensating agent and a buffer for giving a pH value suitable for staining, optionally further adding a salt, which dissociates into ions in aqueous solutions so as to control the electrical conductivity of the aqueous solution at a preferable level, while damaging the cell membranes of erythroblasts contained in said sample; and staining the leukocytes with at least four dyes including Astrazon Yellow 3G and Neutral Red. Thus leukocytes contained in the hematological sample can be classified at least eight groups two each comprising immature granulocytes, one comprising erythroblasts, one comprising basophils, one comprising eosinophils, one comprising lymphocytes, one comprising monocytes and one comprising neutrophils, or nine groups involving one comprising blasts in addition to the above-mentioned eight groups, by assaying a single specimen with a flow cytometer. |
