| 摘要 |
<p>This invention provides a method for discriminating four kinds of nucleic acid bases of DNA at high speed by utilizing chromophores intrinsic to DNA. The method comprises the steps of adding the sample to a polar glassy solvent; reducing a temperature the glassy solution; adding a (n, pi *) quencher as a fluorescence intensifying reagent to the solution; irradiating an excitation light of UV laser beams thereto; measuring lifetimes of fluorescence from the sample; and discriminating the nucleic acid bases of DNA.</p> |
