发明名称 A NOVEL PCR METHOD WITH A SINGLE PRIMER FOR NUCLEIC ACID ANALYSIS
摘要 The invention relates to a process for the amplification of a nucleotide fragment contained in multiple copies of a nucleic acid sample, obtained after digestion of the nucleic acid sample with at least one restriction endonuclease, the nucleotide sequence of the fragment to be amplified being unknown, except for a short nucleotide sequence sufficient to hybridize specifically with an oligonucleotide sequence used as primer in an amplification reaction.
申请公布号 AU2945792(A) 申请公布日期 1993.06.28
申请号 AU19920029457 申请日期 1992.11.25
申请人 KEYGENE N.V. 发明人 GEORGE RUDENKO;JOHN NIJKAMP;JACQUES HILLE
分类号 C12Q1/68 主分类号 C12Q1/68
代理机构 代理人
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