Method for separation of 5-hydroxycreatinine

摘要

The present application provides a method for the separation and determination of 5-hydroxycreatinine (5-HC), comprising the step of subjecting a sample to high performance liquid chromatography (HPLC) separation using a strongly acidic cation-exchange resin and a separation solvent having a pH of 4.1-4.6. By this method practically measurable 5-HC concentrations of as low as 0.02 mu M are achieved, and the method can be performed in a short period of time using only one single separation solvent. This allows for practical applications which have been impossible with the conventional methods, and renders the present method a useful tool in an improved test for the detection of e.g. renal function disorders, such as renal failure, diabetic nephropathy and nephritis, and also as a marker for systemic oxidative stress.