| 摘要 |
FIELD: medicine. ^ SUBSTANCE: the present innovation deals with preparing substances of medicinal preparations. Method for isolating sulfated glycosaminoglycans (sGAG) deals with mechanical purification of biological tissue, washing, hydrolysis of washed out tissue with activated papain at 65°C, precipitation with ethanol, moreover, after mechanical purification one should wash out reduced tissue twice with 0.1M-phosphate buffer heated up to 65°C at pH being 5.8-6.0 at the ratio of 1 portion of tissue - 1.5-2 volumes of buffer to be kept then in new portion of heated buffer for about 15-30 min followed by overcooking in the solution of activated 0.1-0.4%-papain at 65°C for about 6-24 h, overcooked mass should be cooled up to 4°C to be kept at this temperature for 2-24 h; one should remove fats due to filtration and noncooked tissue residues, then during 10-24 h it is necessary to selectively isolate sulfated glycosaminoglycans upon a solid carrier obtained out of bony tissue collagen at particles' size being 0.01-0.35 cu. cm, the carrier should be washed with 0.05-0.1-N-hydrochloric acid, degreased, eluted from the carrier with the solution of 0.6-1.5-N-mineral salt in 0.8-N-acetic acid or 0.01-0.025-N-alkaline solution, moreover, one should precipitate glycosaminoglycans in ethanol to be centrifuged for 15 min at 1500 rot./min at 4°C, the residue should be washed out with ethanol or acetone to be dried and sterilized. The innovation provides the chance to obtain highly purified sGAG for obtaining pharmaceutical substances which could be widely applied for obtaining pharmaceutical preparations and some products of medicinal indication with sGAG being in its composition. ^ EFFECT: higher efficiency. ^ 1 cl, 3 ex, 1 tbl |
