发明名称 |
Process for continuously culturing adherent animal cells |
摘要 |
Adherent 293 human kidney cells are continuously cultured in suspension in serum-free medium. Fresh medium is fed into a culture vessel and spent medium is withdrawn from the vessel. The 293 cells are maintained in small aggregates in suspension at a density of at least 3x106 cells/ml for 30 days. The formation of large cell clumps is prevented by maintaining the concentration of calcium in the serum-free medium at 0.002 mM to 0.3 mM.
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申请公布号 |
US5219752(A) |
申请公布日期 |
1993.06.15 |
申请号 |
US19910730386 |
申请日期 |
1991.07.15 |
申请人 |
TEIJIN, LIMITED |
发明人 |
TAKAZAWA, YOSHIHARU;TOKASHIKI, MICHIYUKI |
分类号 |
C12N5/00;C12N5/08 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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