| 摘要 |
A culture method and system has been realized for in vitro development of bovine embryos from early stage 1- to 2-cell embryos to morula or blastocyst stage. The embryos are placed in a culture system of a buffered balanced salt solution with a carbohydrate energy source medium and bovine oviductal epithelial cells in suspension. Embryo development is enhanced by the addition of bovine or ovine oviductal fluid as well as non-essential amino acids to the system. The method and system can be used to culture genetically manipulated embryos used in transgenic procedures and nuclear transfer. |
