YEAST-DERIVED EPIDERMAL GROWTH FACTOR

摘要

A water-soluble yeast protein of about molecular weight 4770 daltons is isolated having murine Epidermal Growth Factor (mEFG) activity per milligram at least equivalent to 1000 nanograms of mEGF by Elisa Assay Titration. The protein is naturally present in yeast and is free of water-insoluble yeast components. The proten is insoluble in trichloroacetic acid, chloroform, absolute methanol, 95% ethanol, acetone, hexanes, petroleum ether, and 50% methanol-25% ether. The protein is soluble in acidic solutions containing 50% methanol, ethanol or acetone. The protein stimulates the growth and respiration of A431 cells in a culture without serum supplements.