METHOD FOR ASSAYING THE SOD ACTIVITY BY USING A SELF-OXIDIZABLE COMPOUND NECESSARY FOR ITS IMPLEMENTATION, SELF-OXIDIZABLE COMPOUNDS AND PREPARATION THEREOF

摘要

The method for assaying the SOD (super oxide dismutase) activity in a liquid medium is based on the activation of self-oxidization, by SOD activity, of a reactive agent having the general formula (I) wherein either n is 1 or 2, R<1> is -OR<4> or -NR<5>R<6>; R<2> is H, -OR<4>, alkyl (1-6C), or -CH2- or -CH2-CH2-, to form a ring by binding to the phenyl substituant, at meta with respect to R<1>; and R<3> is H, alkyl (1-6C) or -OR<4> (if R<2> is different from -OR<4>); with R<4> being H or alkyl (1-6C); R<5> being H, alkyl (1-6C), -CH2COOH, -C6H5COOH or -C6H5SO3H; and R<6> is H, alkyl (1-6C) or -CH2COOH; or n is 1, R<1> is -OR<4>, R<2> is -CH2-O-, in order to form a ring by bonding of O with the phenyl substituant, at meta with respect to R<1>; and R<3> is H or -OR<4>. Application to assaying the SOD activity in a sample, specially a biological sample, particularly by a single measurement and one calibrating curve.