| 摘要 |
PURPOSE:To measure an attenuated total reflection spectrum without transmitting a sample through a prism by forming a polymer film which holds the sample on the surface of the prism and does not allow the sample to be transmitted through the prism. CONSTITUTION:After a polymer film 22 is closely stuck to the surface of an attenuated total reflection prism 21, a sample 23 is introduced onto the film 22. The sample 23 is prepared by dissolving, for example, a high molecular component 24 such as a protein, etc., like the blood and low molecular component 25, such as urea, etc., in water. Since the polymer film 22 is constituted of a water-soluble polymer material having a large number of hydrophilic groups, such as crosslinked polyacrylic soda, the water contained in the sample and components 24 and 25 dissolved in the water can be sucked in the film 22 by the affinity between polymer ions and water. Moreover, the light 26 made incident to the end face of the prism 21 is propagated through the prism 21 by total reflection and forms a spectrum, because a specific wavelength of the light is absorbed when the light is totally reflected by the interface of the prism 21. |
