| 摘要 |
PURPOSE:To provide the subject new protein obtained from vertebrate bone, having specific molecular weight and specific activity, enabling the prevention and radical cure of the falling off of tooth by alveolar pyorrhea and osteoporosis and the shortening of the treating time of bone fracture and useful as an agent for ossification, etc. CONSTITUTION:Proteins are extracted from vertebrate bones with 4M guanidine hydrochloride/tris-HCl solution after removing minerals from the bones. The extracted liquid is brought into contact with an anion exchange material and the non-absorbed proteins are brought into contact with heparin agarose. The adsorbed proteins are eluted to separate an ossification-inducing active fraction, which is purified by successively treating with butylcellulofine, hydroxyapatite, superlose HR12, ConA agarose and an adsorptive resin to obtain the objective new ossification-inducing protein having molecular weight of about 32kd (measured by SDS polyacrylamide gel electrophoresis in the absence of reducing agent) and about 16kd and 13kd (measured by the similar electrophoresis in the presence of a reducing agent) and a specific activity of about >=7,000 unit/mg-protein measured e.g. by the method of Sampath, et al. |
