| 摘要 |
The present invention provides a method of sequencing both strands of double stranded DNA wherein target DNA is subjected to at least one cycle of PCR using a pair of primers one of which is provided with a functional group for subsequent attachment to a first solid support or is already attached to said support and the other primer is provided with a different functional grouping permitting attachment to a second solid support, reacting the double stranded PCR products with said support or supports and separating the immobilised strands from each other and from the reaction solution, followed by sequencing of each of the said strands. Also provided is a kit for use in such a method. |
