| 摘要 |
PURPOSE:To obtain the subject new protein showing an activity enhancing the chemotactic effect of macrophage chemotactic factor and useful, e.g. as a reagent for immunological study of macrophage or an auxiliary anti-neoplastic agent by culturing lymphoid cells. CONSTITUTION:Lymphoid cells, e.g. hybridoma D6-1 cells obtained by cell fusion between human peripheral lymphocyte and human acute T-cell leukemia cell strain in 45% polyethylene glycol are dispersed in a culture medium and incubated at 37 deg.C for 90min. The cultured solution is subsequently centrifuged and the resultant supernatant is collected. The collected supernatant is purified by the anion-exchange column chromatography in which DEAE-agarose, etc., bufferized by 5mM N-2-hydroxyethylpiperazin-N'-2-ethanesulfonic acid buffer solution (Hz 8.0) is used, thus obtaining the objective new protein capable of enhancing the macrophage chemotactic effect to the macrophage chemotactic factor, produced in lymphocyte and/or lymphoid cells and having about 13000 molecular weight measured by gel filtration method. |
