| 摘要 |
This invention relates to derivatives of dipyrrometheneboron difluoride fluorescent dyes that have an absorption maximum at wavelengths longer than about 525 nm, and are chemically reactive with nucleic acids, proteins, carbohydrates, and other bio- logically derived or synthetic chemical materials. The dyes generally have structure (I), wherein at least one of the substituents R1-R7, is a reactive functional group, and at least one of the substituents R1- R7 contains a bathochromic moiety. The ba- thochromic moiety is an unsaturated organic group, preferably heteroaryl or alkenyl. The remaining substituents, which may be the same or different, are hydrogen, halogen, alkyl (containing 1-5 carbo n atoms), aryl, arylalkyl, or sulfo. The dyes are used to make novel conjugates with members of specific binding pairs that ar e ligands or receptors.
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