MYOGENIC VECTOR SYSTEMS

摘要

The present invention includes a Myogenic vector system (MVS) for the expression of a nucleic acid sequence in myogenic tissue. The MVS contains a promoter, a cassette with the sequence of interest, a 3' untranslated region (3' UTR) and contiguous noncoding region (NCR). Further enhancements can include the addition of a leader sequence, intron sequence, initiation codon and specific restriction endonucleases. To facilitate uptake and myogenic expression, the MVS can be coated with histones and a DNA initiation complex composed of a serum response factor, transcription initiation factor and a transregulatory factor attached to the promoter by interaction with the serum response element and TATA box. The MVS can be used for a variety of purposes including gene replacement, vaccine production, vaccination, treatment of disease and muscle atrophy. When the MVS is injected into a myogenic system it will produce the polypeptide from the sequence of interest.