发明名称 |
METHOD FOR ASSAYING FOR A SUBSTANCE THAT AFFECTS AN SH2-PHOSPHORYLATED LIGAND REGULATORY SYSTEM |
摘要 |
A method for assaying a medium for the presence of a substance that affects an SH2-phosphorylated ligand regulatory system. The method employs an SH2-like domain or a subdomain thereof and a phosphorylated ligand. The phosphophorylated ligand is capable of interacting with the SH2-like domain or a subdomain thereof to form an SH2- phosphorylated ligand complex. The SH2-like domain or subdomain and/or the phosphorylated ligand are present in a known concentration. The SH2-like domain or a subdomain thereof and the phosphorylated ligand are incubated with a substance which is suspected of affecting an SH2- phosphorylated ligand regulatory system. The method is carried out under conditions which permit the formation of the SH2-phosphorylated ligand complex. SH2-phosphorylated ligand complex, free SH2-like domain or subdomains thereof, or non-complexed phosphorylated ligand are assayed. The invention also relates to an isolated SH2- phosphorylated ligand complex; a method of using an isolated SH2-like domain or a subdomain thereof to screen for phosphorylated ligands which are active in an SH2- phosphorylated ligand regulatory system; a method of using an isolated SH2-like domain or a subdomain thereof to regulate the interaction of a signalling protein with a related phosphorylated ligand; and a pharmaceutical composition comprising an isolated SH2-like domain or a subdomain thereof for use as an agonist or antagonist of the interaction of a signalling protein with a related phosphorylated ligand.
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申请公布号 |
CA2054602(A1) |
申请公布日期 |
1993.05.01 |
申请号 |
CA19912054602 |
申请日期 |
1991.10.31 |
申请人 |
MOUNT SINAI HOSPITAL CORPORATION |
发明人 |
PAWSON, ANTHONY |
分类号 |
C07K14/47;C12N9/12;G01N33/50;G01N33/53;G01N33/68;G01N33/74;(IPC1-7):G01N33/68 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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