METHOD FOR FREEZING ENGRAFTING CELLS

摘要

2122140 9307745 PCTABS00021 The present invention provides a method for preparing engrafting cells for future use, comprising the steps of (a) purifying engrafting cells; (b) concentrating the purified engrafting cells; (c) resuspending the concentrated cells to a concentration of about 10 x 106 to 40 x 106 cells/ml in a solution that substantially maintains cell viability during freezing; and (d) freezing the resuspended cells under a first set of conditions which substantially maintains cell viability.