| 摘要 |
<p>Methods of diagnosing borreliosis, Lyme disease, by detecting the presence of a Borrelia burgdorferi organism in a specimen from an animal, including a human, by the use of PCR-DNA analysis, where the primers are subsequences from OspA and OspB DNA sequences from any of three B. burgdorferi species I, II and III, such as OspA and OspB DNA sequences from one of the following B. burgdorferi strains: B31, Ip90 (or Iper90) and ACAI, respectively. The method may be used for detecting the presence of B. burgdorferi in a specimen irrespective of the species and strain of the B. burgdorferi organism proposed to be in the specimen, but may also be used for detecting specific strains, depending on the primers chosen. Vaccines comprising polypeptides capable of evoking an immune response against OspA or OspB of B. burgdorferi organisms for active immunization, the polypeptides preferably being lipidated. DNA sequences encoding the polypeptides, preferably also encoding a lipoprotein signal peptide, e.g. a signal peptide comprising L-x-y-C in the C-terminal region and a method for producing the polypeptide.</p> |
