发明名称 |
PARTICLE-MEDIATED TRANSFORMATION OF MAMMALIAN UNATTACHED CELLS |
摘要 |
A method of genetically transforming mammalian unattached cells is disclosed. The method begins by preparing copies of a nucleic acid construct and coating these copies onto biologically inert carrier particles. Mammalian unattached cells are isolated in a liquid suspension. The cell suspension is placed on a target surface, wherein the liquid is spread to a thin film on the target surface. In an alternative embodiment of the present invention, the liquid is spread onto a porous surface. The cells are bombarded with the construct-coated particles in such a fashion that some particles lodge in the interior of at least some of the cells. The existence and expression of the construct within the cell is verified. |
申请公布号 |
WO9308270(A1) |
申请公布日期 |
1993.04.29 |
申请号 |
WO1992US08806 |
申请日期 |
1992.10.15 |
申请人 |
AGRACETUS, INC. |
发明人 |
YANG, NING-SUN;SWAIN, WILLIAM, F.;BURKHOLDER, JOSEPH, K.;FULLER, DEBORAH, L. |
分类号 |
C12N5/10;C12N15/09;C12N15/87 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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